论文部分内容阅读
与皮肤及软骨相比,骨骼肌对缺血更敏感。缺血肌肉高能磷酸化合物储存的耗竭,是决定其是否发生坏死的重要因素。本实验调查磷酸(烯醇)丙酮酸(PEP)和三磷酸腺苷(ATP)对常温下缺血骨骼肌瓣成活的作用。50只成年白兔,制成带蒂股直肌瓣和游离背阔肌瓣模型各25例。每种肌瓣分成3组,前两组各10例分别于37℃和20℃下完全缺血4小时;在恢复血供之前,分别以2ml Hanks平衡盐液和2 ml含有200μmol PEP及6μmol ATP的平衡液灌注15分钟。第3组各5例,作为未缺血对照。于术前、肌瓣恢复血供后0、2、24、96小时分别由耳静脉取血样测定肌酸激酶(CK)、
Skeletal muscle is more ischemic than skin and cartilage. The depletion of ischemic muscle stores of high-energy phosphate is an important determinant of necrosis. This experiment investigates the effect of phosphoenolpyruvate (PEP) and adenosine triphosphate (ATP) on the survival of ischemic skeletal muscle flaps at room temperature. Fifty adult white rabbits were made into pedicled rectus abdominis flap and free latissimus dorsi flap model in 25 cases. Each muscle flap was divided into three groups. The first two groups of 10 patients were completely ischemia at 37 ℃ and 20 ℃ for 4 hours respectively. Before the recovery of blood supply, 2 ml Hanks balanced salt solution and 2 ml PBS containing 200 μmol PEP and 6 μmol ATP Balancing solution for 15 minutes. Group 3 each 5 cases, as not ischemic control. Preoperative, muscle flap recovery blood supply 0,2,24,96 hours were taken from the ear vein blood creatine kinase (CK)