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目的:既往研究显示SePP1具有一定的抗氧化作用,而随着年龄的增加机体逐步出现一个慢性低氧、炎症状态,我们通过4%O2浓度体外培养大鼠脂肪前体细胞模拟其体内的低氧状态,进而观察常氧(21%O2)及低氧(4%O2)状态下大鼠脂肪前体细胞中炎症因子(IL-6,MCP-1,SePP1)水平的变化及不同状态下硒蛋白SePP1水平的变化。方法:取6-8周SD大鼠肾周脂肪前体细胞,分别于常氧(21%O2)及低氧(4%O2)状态下进行体外培养,诱导分化后采用油红O染色进行鉴定,至第三代后,分别采用PCR及Western Blot技术检测两种状态下脂肪前体细胞中IL-6,MCP-1,SePP1基因及蛋白表达的不同变化,同时观察不同氧浓度对脂肪前体细胞增殖的影响。结果:4%氧浓度状态下培养的脂肪前体细胞中IL-6,MCP-1的基因及蛋白表达均明显高于正常氧浓度下的脂肪前体细胞,而SePP1的基因及蛋白表达均下降,且低氧状态下脂肪前体细胞增殖较常氧状态下加快。结论:低氧培养可进一步使机体内脏脂肪组织堆积加重,造成脂肪前体细胞的炎症状态,并且可导致SePP1的表达下降,而SePP1具有一定的抗氧化作用,与机体动脉粥样硬化等心血管疾病的发生、发展有一定的关联,本实验结论为通过干预体内SePP1的水平为靶点治疗动脉粥样硬化提供了一定的实验依据,为进一步研究SePP1在低氧状态下对动脉粥样硬化的影响及作用机制提供了一定的试验基础。
OBJECTIVE: Previous studies have shown that SePP1 has a certain anti-oxidant effect. With the gradual emergence of a chronic hypoxia and inflammatory state in the body, we cultured rat adipose precursor cells at a concentration of 4% O2 to simulate hypoxia in vivo The changes of inflammatory cytokines (IL-6, MCP-1, SePP1) in rat adipose precursor cells under normoxic (21% O2) and hypoxic (4% O2) Changes in SePP1 levels. Methods: Peripheral adipose precursor cells from 6-8 weeks old SD rats were cultured in vitro under the condition of normoxia (21% O2) and hypoxia (4% O2), respectively. The differentiated cells were identified by oil red O staining After the third generation, the changes of IL-6, MCP-1, SePP1 gene and protein expression in adipose precursors were detected by PCR and Western Blot respectively. At the same time, Effect of cell proliferation. Results: The gene and protein expressions of IL-6 and MCP-1 in adipose precursor cells cultured at 4% oxygen concentration were significantly higher than those at normal oxygen concentration, whereas the gene and protein expression of SePP1 decreased , And the proliferation of adipose precursor cells under normoxia state is faster than normoxia state. Conclusion: Hypoxic culture can further increase the accumulation of visceral adipose tissue, leading to the inflammatory state of adipose precursor cells and lead to the decrease of SePP1 expression. However, SePP1 has a certain anti-oxidant effect, which is related to cardiovascular diseases such as atherosclerosis Disease development, there is a certain correlation between the experimental conclusions for the intervention of the level of SePP1 in vivo as a target for the treatment of atherosclerosis provided some experimental basis for further study SePP1 hypoxia state atherosclerosis Influence and mechanism of action provides some experimental basis.