论文部分内容阅读
该研究通过对金银花叶片进行不同时间(1,2,3 d)与不同浓度(20,40,60,80,100μmol·L-1)的5-氮杂胞苷处理,探索5-氮杂胞苷影响黄酮合酶FNSⅡ催化木犀草素和木犀草苷合成的机制。首先对Lj FNSⅡ1.1,Lj FNSⅡ2.1进行克隆;其次UPLC-MS/MS测定金银花叶中木犀草素与木犀草苷的含量,以及Real-Time PCR分析Lj FNSⅡ1.1,Lj FNSⅡ2.1的表达水平,结果表明Lj FNSⅡ1.1,Lj FNSⅡ2.1基因的表达水平与木犀草素的含量变化趋势大体一致,但与木犀草苷的含量变化相关性不显著;同时发现二者之间的表达水平略有差异。研究结果表明,5-氮杂胞苷处理金银花叶片后,Lj FNSⅡ1.1,Lj FNSⅡ2.1的表达水平上升,从而调控木犀草素和木犀草苷的含量升高,为研究Lj FNSⅡ1.1,Lj FNSⅡ2.1催化木犀草素和木犀草苷合成机制提供技术支撑和理论基础。
In this study, we treated 5-azacytidine of Lonicera japonica Thunb. By different time (1, 2 and 3 days) and 5-azacytidine (20, 40, 60, 80 and 100μmol·L-1) The mechanism of flavone synthase FNS Ⅱ catalyzing the synthesis of luteolin and luteolin was studied. Firstly, Lj FNSII1.1 and Lj FNSII2.1 were cloned. Secondly, the contents of luteolin and luteolin in Lonicera japonica Thunb. Were determined by UPLC-MS / MS. Real-time PCR analysis of Lj FNSII1.1, Lj FNSII2.1 The results showed that the expression levels of Lj FNSII1.1 and Lj FNSII2.1 gene were almost the same as those of luteolin, but not with the content of luteolin The level is slightly different. The results showed that Lj FNSII1.1, Lj FNSII2.1 expression increased after 5-azacytidine treatment of honeysuckle leaves, thereby regulating the luteolin and luteolin content increased, for the study of Lj FNSII1.1, Lj FNS Ⅱ 2.1 catalytic luteolin and luteolin synthesis mechanism to provide technical support and theoretical basis.