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目的：观察内毒素攻击诱导小鼠肝细胞凋亡及与肿瘤坏死因子α（ＴＮＦα）的关系。方法：用精制内毒素腹腔注射于实验小鼠，分别于不同时间点测其血清中ＴＮＦα、谷丙转氨酶（ＧＰＴ）和乳酸脱氢酶（ＬＤＨ）含量，同时取肝脏制成组织切片进行ＨＥ和末端脱氧核糖转移酶介导的生物素化脱氧尿嘧啶缺刻标记技术（ＴＵＮＥＬ）染色，分别于光镜及荧光显微镜下观察肝细胞凋亡情况。结果：在内毒素攻击下，小鼠血清ＴＮＦα于９０分钟时首先达到峰值（１２９１．５０±１３４．４０）ｎｇ／Ｌ，其后即下降；２小时时肝细胞出现明显凋亡现象，肝组织坏死及血清酶学改变于４小时才明显产生。结论：内毒素首先诱导ＴＮＦα等释放，而ＴＮＦα及其诱生的其它细胞因子可使肝细胞发生凋亡
Objective: To observe the effect of endotoxin challenge on hepatocyte apoptosis and its relationship with tumor necrosis factor α (TNFα) in mice. Methods: The mice were injected intraperitoneally with purified endotoxin. The levels of TNFα, alanine aminotransferase (GPT) and lactate dehydrogenase (LDH) were measured at different time points. At the same time, the liver was made into tissue sections for HE and End Deoxyribo-transferase-mediated biotinylated deoxyuracil nick labeling (TUNEL) staining, respectively, under light microscope and fluorescence microscope to observe the apoptosis of hepatocytes. Results: Under endotoxin challenge, the serum TNFα peaked at 1290.50 ± 134.40 ng / L at first, and then decreased afterwards. At 2 hours, the apoptosis of hepatocytes was obvious, Necrosis and serum enzyme changes were not apparent until 4 hours. CONCLUSION: Endotoxin first induces the release of TNFα and so on, while TNFα and other cytokines induced by it induce the apoptosis of hepatocytes