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目的研究低压缺氧预处理对加速度环境下脑细胞病理生理变化的影响,探讨低压缺氧预处理的保护机制。方法 24只雄性SD大鼠随机分为3组(n=8),Con组为空白对照组,HHP+10 Gz组为低压缺氧预处理后暴露10 Gz加速度组,10 Gz组为直接暴露10 Gz加速度组,应用Morris水迷宫检测各组大鼠学习记忆能力,取大鼠脑组织检测SOD、CAT、GSH-PX、GSH、MDA和HSP-70含量。结果登台潜伏期:Con组大鼠全部登台,登台潜伏期为9.828±1.291s;HHP+10 Gz组有2只大鼠登台,登台潜伏期分别为31.45s和42.78s;10 Gz组仅有1只大鼠成功登台,登台潜伏期为92 s;SOD、GSH-PX、HSP-70:Con组大于10 Gz组,HHP+10 Gz组大于10 Gz组;CAT含量各组间差异无统计学意义;GSH:Con组大于HHP+10 Gz组,Con组大于10 Gz组,HHP+10 Gz组大于10 Gz组;MDA:Con组小于HHP+10 Gz组,Con组小于10 Gz组。结论低压缺氧预处理可改善加速度对大鼠学习记忆能力的损害,降低脑组织氧化损伤,其机制与增强大鼠体内抗氧化酶活性有关。
Objective To study the effects of hypobaric hypoxia preconditioning on the pathophysiological changes of brain cells under acceleration environment and to explore the protective mechanism of hypobaric hypoxic preconditioning. Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups (n = 8). Con group was blank control group. HHP + 10 Gz group was exposed to 10 Gz acceleration after hypobaric hypoxic preconditioning and 10 Gz group was exposed to 10 Gz acceleration group. Morris water maze was used to detect the learning and memory abilities of rats in each group. The contents of SOD, CAT, GSH-PX, GSH, MDA and HSP-70 in brain tissues were determined. Results The incubation period was all on stage in Con group, with an incubation period of 9.828 ± 1.291s. Two rats in HHP + 10 Gz group were on stage with latency of 31.45s and 42.78s respectively. Only one rat in 10 Gz group (P <0.05), and the latent period was 92 s. The SOD, GSH-PX, HSP-70: Con group was greater than 10 Gz group, HHP + 10 Gz group was larger than 10 Gz group; CAT content was no significant difference among groups; Group HHP + 10 Gz was larger than that of HG + 10 Gz, Group H was larger than 10 Gz, Group HHP + 10 Gz was larger than Group 10 Gz, Group H was less than HG + 10 Gz, Group H was less than 10 Gz. Conclusion Hypobaric hypoxia preconditioning can improve the impairment of learning and memory in rats and decrease the oxidative damage of brain tissue. Its mechanism is related to the enhancement of antioxidase activity in rats.