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利用双抗体酶联免疫吸附测定法(DAS-ELISA)对采自福建省51份甘薯叶片进行检测,结果显示51份样品中有24份SPFMV呈阳性,阳性率为47.06%,其中泉州样品SPFMV阳性率最高,为71.40%,其次为南安和龙岩的样品,阳性率均为50%。根据GenBank中公布的SPFMV外壳蛋白(CP)基因序列保守区域设计了1对特异性引物,通过RT-PCR反应程序的优化建立了能检测SPFMV的检测方法。该检测方法能够扩增出SPFMV特异性片段,片段大小为441bp,测序结果表明,SPFMV序列与参考序列的同源性92%~97%。
The results showed that SPFMV was positive in 24 out of 51 samples, the positive rate was 47.06%, of which Quanzhou samples were positive for SPFMV by using double antibody enzyme-linked immunosorbent assay (DAS-ELISA) The highest rate was 71.40%, followed by the samples of Nanan and Longyan. The positive rates were both 50%. A pair of specific primers was designed according to the conserved region of the CP gene of SPFMV published in GenBank. The detection method of SPFMV was established by optimizing the RT-PCR reaction program. The detection method can amplify the SPFMV specific fragment with a fragment size of 441 bp, and the sequencing result shows that the homology between the SPFMV sequence and the reference sequence is 92% -97%.