论文部分内容阅读
目的探讨肉苁蓉水提液对猪早期体外发育胚胎中一氧化氮浓度的影响,以研究肉苁蓉促进猪胚胎发育的药理作用。方法将采集的猪卵母细胞随机分为试验组和对照组,试验组在胚胎培养时在基础液中加入50μg/mL肉苁蓉水提液。将培养至囊胚阶段的胚胎取出,用Griess试剂法测定囊胚细胞中NO含量。结果细胞中NO含量显著低于对照组(P<0.05)。结论肉苁蓉水提液减少了胚胎细胞中NO含量,这可能是其促进细胞体外发育的作用机理之一。
Objective To investigate the effect of Cistanche deserticola extract on the concentration of nitric oxide in early-stage embryos of pigs in order to study the pharmacological effects of Cistanche on the development of pig embryos. Methods The collected pig oocytes were randomly divided into experimental group and control group. In the experimental group, 50 μg/mL Cistanche water extract was added to the base fluid during embryo culture. The embryos that were cultured to the blastocyst stage were removed and the NO content in the blastocyst cells was determined by Griess reagent method. Results The NO content in the cells was significantly lower than that in the control group (P<0.05). Conclusion The water extract of Cistanche deserticola reduced the NO content in embryonic cells, which may be one of the mechanisms of its promotion of cell development in vitro.