论文部分内容阅读
采用基于捕获与连接的探针式PCR(capture and ligation probe-PCR,CLIP-PCR)矩阵拆分混样法和镜检两种方法对100份临床样本进行检测,综合评价CLIP-PCR矩阵拆分混样法对疟原虫的检测效果。从云南省疟疾参比实验室标本库选取93份阴性样本和7份阳性样本,随机混合,采用双盲法进行CLIP-PCR矩阵拆分混样检测,并与镜检法的结果对比分析,通过检出率、灵敏度、特异度、检测时间及检测成本等进行综合评价。7份镜检阳性样本被全部检出,检出率为100%。以镜检为金标准,CLIP-PCR矩阵拆分混样检测灵敏度和特异度均为100%。全部样本的检测时间为5.0 h,较镜检的33.3 h缩短85.0%;检测成本为300元,较镜检的1 000元降低了70.0%。
A total of 100 clinical samples were detected by PCR-based capture-ligation probe-PCR (CLIP-PCR) matrix hybridization and microscopy. The results of CLIP-PCR matrix resolution Mixed method of detection of Plasmodium effect. Ninety-three negative and seven positive samples were selected from the malaria reference laboratory specimen library in Yunnan Province. Randomly mixed, the double-blind method was used to detect the mixed samples of CLIP-PCR matrix and compared with the results of microscopic examination. Detection rate, sensitivity, specificity, testing time and testing costs for a comprehensive evaluation. Seven positive samples were all detected microscopy, the detection rate was 100%. With the microscopic examination as the gold standard, the sensitivity and specificity of the CLIP-PCR matrix disassembly mixed detection were both 100%. The detection time of all samples was 5.0 h, which was 85.0% shorter than that of 33.3 h. The detection cost was 300 Yuan, which was 70.0% lower than the 1,000 Yuan of microscopic examination.