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作者以~(125)碘标记不含apoE的人HDL_3为配体,采用简便的聚乙二醇(PEG)沉淀分离法,建立了纯化的大鼠肝细胞膜HDL受体分析法。最终浓度为12.5％的PEG能有效沉淀~(125)I-HDL_3-膜受体复合物,总结合中94％以上为特异性结合(非特异性结合在有过量25倍的非标记HDL_3存在下
In this study, HDL-3 was purified and purified by polyethylene glycol (PEG) precipitation method using ~ (125) iodine-labeled apoE-free human HDL_3 as a ligand. PEG at a final concentration of 12.5% effectively precipitated the ~ (125) I-HDL_3-membrane receptor complex with more than 94% of the total binding specific binding (nonspecifically bound in the presence of 25-fold excess of unlabeled HDL_3