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“邱”ד皇后”菠萝杂种胚愈伤组织已分化成苗。将带杂种胚的种子培养在含有苄基腺嘌呤或吲哚丁酸的培养基上,诱发胚芽愈伤组织。愈伤组织从根部形成,最终包裹了整个胚。将原始愈伤组织进行多次中间培养,就可促使它继续生长。中间培养的愈伤组织中,发现有芽分化。将从芽丛上分离的单株移至基本培养基上培养。从一个杂种胚平均可获得20~25株发育完全的植株。
“Qiu” × “Queen” pineapple hybrid embryo callus has differentiated into seedlings. The seeds with hybrid embryos are cultured on a medium containing benzyladenine or indole butyric acid to induce germ callus. Callus is formed from the root, eventually wrapping the entire embryo. The original callus multiple intermediate culture, you can make it continue to grow. Intermediate callus, found bud differentiation. Individual plants isolated from shoots were transferred to basal medium and cultured. An average of 20 to 25 well-developed plants are obtained from one hybrid embryo.