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目的探讨环氧化酶2(COX-2)抑制剂NS-398对舌鳞癌基质金属蛋白酶2(MMP-2)蛋白表达的影响。方法将舌鳞癌细胞株Tca8113分为对照组(A组)和NS-398 20、40、80μmol/L加药组(分别为B1、B2、B3组);荧光免疫组化和Western blot法检测各组MMP-2蛋白表达情况。结果 B1、B2、B3组MMP-2蛋白表达呈递减趋势,且均较A组明显降低(免疫组化法:366073.100±116272.561、241739.000±12320.270、120672.100±5976.391vs.591518.000±17356.450;Western blot法:0.620±0.084、0.425±0.058、0.172±0.025vs.0.950±0.124)(P<0.05)。结论 NS-398在Tca8113细胞中能抑制MMP-2蛋白表达,并且MMP-2蛋白表达随着药物浓度的增加而减少。
Objective To investigate the effect of COX-2 inhibitor NS-398 on the expression of matrix metalloproteinase-2 (MMP-2) in tongue squamous cell carcinoma. Methods The tongue squamous carcinoma cell line Tca8113 was divided into control group (group A) and NS-398 20, 40, and 80μmol / L dosing group (B1, B2 and B3 respectively). Fluorescence immunohistochemistry and Western blot The expression of MMP-2 protein in each group. Results The expressions of MMP-2 protein in B1, B2 and B3 groups decreased gradually, which were significantly lower than those in group A. Immunohistochemistry: 366073.100 ± 116272.561,241739.000 ± 12320.270,120672.100 ± 5976.391 vs.591518.000 ± 17356.450; Western blot: 0.620 ± 0.084, 0.425 ± 0.058, 0.172 ± 0.025 vs. 0.950 ± 0.124) (P <0.05). Conclusion NS-398 can inhibit the expression of MMP-2 protein in Tca8113 cells, and the expression of MMP-2 protein decreases with the increase of drug concentration.