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睫状神经营养因子(Ciliary neurotrophic factor,CNTF)是第一个被发现可促进离体和在体运动神经元存活、对神经系统正常发育和受损神经的再生修复有重要作用的神经因子,主要存在于星形胶质细胞和施旺细胞.人CNTF基因定位于11号染色体长臂的近例端,其基因结构不与神经营养素家族同源也不含信号肽.我们发现大鼠坐骨神经再生过程中CNTF的基因表达下调,提示它的表达量与损伤后胶质细胞的增殖未必匹配.国外已重组了人CNTF,国内尚未见报道.由于我们没有人系统神经的cDNA文库,故用大鼠CNTFcDNA探针来检测人视神经胶质细胞瘤和人脑胶质母细胞瘤细胞系中是否有人CNTF的表达,再用反转录PCR克隆出人CNTF cDNA,目的是获得重组的人CNTF蛋白.
Ciliary neurotrophic factor (CNTF) is the first nerve factor that has been found to play an important role in promoting the survival and survival of motor neurons in vitro and in the normal development of the nervous system and the regenerative repair of damaged nerves. The main Exist in astrocytes and Schwann cells.The human CNTF gene is located at the near end of the long arm of chromosome 11. Its genetic structure is not homologous with the neurotrophin family nor the signal peptide.We found that the rat sciatic nerve regeneration process In CNTF gene expression down, suggesting that the expression of glial cells after injury and its proliferation may not match the foreign CNTF has been recombinant, has not been reported in China.Because we do not have a human nervous system cDNA library, so rat CNTF cDNA Probe to detect the presence of human CNTF in human glioma and human glioblastoma cell lines, and then human CNTF cDNA was cloned by reverse transcription PCR in order to obtain recombinant human CNTF protein.