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在临床工作中,由于病理诊断的需要,我们经常需要对冷冻组织进行固定并做免疫组化染色。然而由于多种因素的影响,冷冻组织的免疫组化结果相比常规固定组织往往不够理想,一定程度上影响了病理诊断的准确性。常规的免疫组化组织由于中性甲醛固定造成蛋白质交联,使得部分或大部分抗原决定簇被封闭,一般需要通过抗原修复才能使抗原表位重新暴露并与抗体有效结合,因此抗原修复成为免疫组
In clinical practice, due to the need for pathological diagnosis, we often need to fix the frozen tissue and do immunohistochemistry. However, due to a variety of factors, immunohistochemical results of frozen tissue is often not ideal compared with conventional fixed tissue, to a certain extent, affect the accuracy of pathological diagnosis. Conventional immunohistochemistry due to neutral formaldehyde fixation caused by protein cross-linked, making some or most of the epitopes are blocked, usually need to go through the antigen repair in order to re-expose the antigen epitope and effective binding with the antibody, the antigen repair becomes immune group