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目的 检测Fas基因在各类人卵巢组织标本和卵巢癌细胞系中的表达并分析其对肿瘤发生的意义。方法 应用免疫组织化学染色和Western印迹杂交技术 ,对 8例非瘤卵巢组织、18例良性和 2 3例卵巢癌标本以及 3株卵巢癌细胞系中Fas基因的表达情况加以分析 ,观察抗Fas抗体对卵巢癌细胞体外生长的影响。结果 Fas在非瘤卵巢组织中的阳性表达率是 12 5 % ,在良性肿瘤为 89% ,在浆液性囊腺癌和粘液性囊腺癌分别为10 0 %和 86 % ;3株卵巢癌细胞系Fas均呈阳性表达。Western印迹杂交显示 :卵巢癌细胞产生的Fas蛋白以分子质量 40ku的可溶型 (sFas)为主 ,伴少 (痕 )量的 43ku膜型 (mFas)。用最高浓度为 2 0 0 μg/L的抗Fas抗体处理 3株卵巢癌细胞系 ,未能抑制靶细胞生长和诱导凋亡。结论 sFas普遍存在于卵巢肿瘤和癌细胞系。缺乏mFas可能是影响卵巢癌细胞凋亡和 (或 )化疗耐受性的遗传学因素之一
Objective To detect the expression of Fas gene in various human ovarian tissues and ovarian cancer cell lines and to analyze its significance for tumorigenesis. Methods Immunohistochemical staining and Western blotting were used to analyze the expression of Fas gene in 8 non-neoplastic ovarian tissues, 18 benign and 23 ovarian cancer specimens and 3 ovarian cancer cell lines. The anti-Fas antibody Effect on ovarian cancer cell growth in vitro. Results The positive rate of Fas in non-neoplastic ovarian tissues was 125%, 89% in benign tumors and 100% and 86% in serous cystadenocarcinoma and mucinous cystadenocarcinoma respectively. Three ovarian cancer cells Fas Fas positive expression. Western blotting showed that the Fas protein produced by ovarian cancer cells was predominantly soluble in 40 ku of soluble form (sFas) with a small amount of 43 ku membrane (mFas). Three ovarian cancer cell lines treated with anti-Fas antibody at the highest concentration of 200 μg / L failed to inhibit target cell growth and induce apoptosis. Conclusion sFas is widespread in ovarian tumors and cancer cell lines. Lack of mFas may be one of the genetic factors that affect ovarian cancer cell apoptosis and / or chemoresistance