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三七总皂苷为三七主要的药用活性成分,但因其产量较低,使其药用价值很难得到推广。三七皂苷和甾醇分别经达玛烯二醇合酶(DS)和环阿屯醇合酶(CAS)的催化共同的前体物质2,3-氧化鲨烯生成。利用Gateway技术建立了CAS基因的RNA干扰(RNAi)表达载体,并利用农杆菌介导法将其转移并整合至三七基因组中,由此方法抑制CAS的表达,阻断植物甾醇的合成,间接增加三七皂苷的产量。
Panax notoginseng saponins is the main medicinal active ingredient of Panax notoginseng, but its medicinal value is hard to be popularized due to its low yield. Notoginsenosides and sterols were generated from 2,3-squalene, the common precursor catalyzed by damasene synthase (DS) and cycloartenol synthase (CAS), respectively. The RNA interference (RNAi) expression vector of CAS gene was established by using Gateway technology and was transferred and integrated into genome of Panax notoginseng by Agrobacterium tumefaciens method. This method inhibited the expression of CAS and blocked the synthesis of phytosterol, indirectly Increase notoginsenoside production.