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紫外光在临床医学上的众多应用中最值得关注的是紫外光照射自血回输疗法,但其具体机制尚未清楚.基于倒置荧光显微镜光路,在单细胞水平上,分别以连续式和间隔式365 nm长波紫外光辐照射血液中数量最多的白细胞-嗜中性粒细胞,检测其对细胞内钙离子浓度的影响.结果表明,相对低剂量的长波紫外光辐照会使胞内钙浓度升高,而较高剂量的紫外光可导致胞内钙浓度下降,甚至低于起始钙浓度.此外,胞外无钙的情况下,紫外仍可引起胞内钙升高,说明紫外引起胞内钙库释放导致胞内钙浓度升高.为探讨紫外光照射自血回输疗法的作用机理提供一定的实验支持.
However, the specific mechanism of UV-light irradiation is not clear.Using inverted light microscopy optical path, at the single-cell level, the continuous and intermittent 365 nm long-wave ultraviolet (UV) light was used to irradiate leukocyte-neutrophil in the blood to detect its effect on intracellular Ca2 + concentration.The results showed that the relatively low dose of UVB irradiation could increase intracellular calcium concentration Increased, and higher doses of ultraviolet light can lead to intracellular calcium concentration decreased, or even lower than the initial calcium concentration.In addition, extracellular calcium in the case, UV can cause intracellular calcium increased, indicating that UV-induced cell The release of intracellular calcium leads to an increase of intracellular calcium concentration.To provide some experimental support for exploring the mechanism of UVB irradiation.