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介绍了高效液相色谱定量分析之绝对分析方法的理论推导。简要说明了其在氨基酸、芳香胺等的定量分析中的应用和用于研究不同的流动相组成对化合物摩尔吸光系数的影响
The theoretical derivation of the absolute analytical method for quantitative analysis of high performance liquid chromatography (HPLC) is introduced. Its application in the quantitative analysis of amino acids, aromatic amines, etc. and its effects on the molar extinction coefficients of the compounds are briefly described