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2016年6月,广西邕宁某场有20%的阳江鹅在喙、眼和脚出现赘肉状痘癍,临床无死亡病例。为了研究此病的病原和基因特征,采集痘癍样本、制备病理组织切片进行显微镜观察、并利用透射电镜进行形态学分析以及TaqMan荧光定量PCR检测,证实其病原为禽痘病毒属(Avipoxvirus,APV)成员,命名为YNE。通过对病毒核芯蛋白P4b编码基因(fpv167)和DNA聚合酶Popr编码基因(fpv094)的部分序列串联进行比对,发现该毒株与1991年分离于北美林鸳鸯的APV高度同源,属于A5.2基因分支。进一步序列分析发现,该毒株与同期发生在该场的麻鸭源APV相应序列的同源性可达99.9%,初步证实该病毒可以在鸭鹅间跨种传播。
In June 2016, 20% of Yangjiang geese in a farm in Suining, Guangxi province were present with no cellulitis in the beak, eyes and feet. There were no clinical deaths. In order to study the etiological and genetic characteristics of the disease, pox samples were collected to prepare histological sections for microscopic observation. Transmission electron microscopy was used for morphological analysis and TaqMan real-time PCR detection to confirm that the pathogen was Avipoxvirus (APV) ) Member, named YNE. The sequence of virus core protein P4b encoding gene (fpv167) and DNA polymerase gene coding sequence (fpv094) was sequenced and found to be highly homologous to the APV isolated from North American wood ducks in 1991 and belonged to A5 .2 gene branch. Further sequence analysis revealed that the homology of this strain with the corresponding sequence of APH in ducks at the same period reached 99.9%. It was initially confirmed that the virus could cross-species between ducks and geese.