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目的对3例疑难恶性疟病例进行实验室快速检测,评价快速检测方法的临床应用价值。方法以2014年6月至2015年5月3例因不明原因发热入院患者为研究对象,采用免疫胶体金快速检测法和显微镜检法分别检测患者血液疟原虫,采用核酸荧光染料和FCM检测白细胞计数(WBC)、红细胞计数(RBC)、血色素(Hgb)、血小板计数(PLT)等血常规指标,采用酶法检测患者C反应蛋白(CRP)、直接胆红素(DBIL)、总胆红素(TBIL)和谷丙转氨酶(ALT)等水平。结果 3例不明原因发热患者以畏寒、发热为主要症状,均出现血小板降低、CRP升高,和不同程度肝功能损害。胶体金快速检测和血涂片疟原虫显微镜检结果相同,均为恶性疟原虫感染。结论免疫胶体金法快速检测有助于临床快速诊断疟原虫感染。
Objective To rapidly test 3 cases of falciparum falciparum in laboratory and evaluate the clinical value of rapid detection. Methods From June 2014 to May 2015, 3 patients with fever due to unknown causes were enrolled in this study. Plasmodium falciparum was detected by immunogold assay and microscopy. The white blood cell count (WBC), RBC, Hgb and PLT were detected by enzyme-linked immunosorbent assay (ELISA). CRP, DBIL and total bilirubin TBIL) and alanine aminotransferase (ALT) and other levels. Results In 3 patients with fever of unknown origin, chills and fever were the main symptoms. Both of them showed thrombocytopenia, elevated CRP, and liver dysfunction. Rapid detection of colloidal gold and blood smear Plasmodium microscopy results are the same, are Plasmodium falciparum infection. Conclusion Immune colloidal gold method for rapid detection of the clinical rapid diagnosis of Plasmodium infection.