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目的探索青春型双歧杆菌的 DNA 对巨噬细胞 PKC 家族和 NF-kB 的影响。方法以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞 PKC_α、PKC_βⅠ、PKC_βⅡ、PKC_γ、PKC_ε和 PKC_ζ的含量,以细胞免疫化学方法检测巨噬细胞 NF-kB 的阳性细胞密度。结果双歧杆菌 DNA 注射组小鼠腹腔巨噬细胞 PKC_α和 PKC_βⅡ的平均荧光强度明显高于对照组(P<0.01),而 PKC_βⅠ、PKC_γ、PKC_ε和 PKC_ζ的平均荧光强度在两组间则无明显差异(P>0.05)。双歧杆菌 DNA 注射组巨噬细胞 NF-kB 的阳性细胞密度显著高于对照组(P<0.01)。结论青春型双歧杆菌的 DNA 可通过活化 PKC_α、PKC_βⅡ和 NF-kB 来激活巨噬细胞。
Objective To explore the effect of DNA of Bifidobacterium adolescent on PKC family and NF-κB in macrophages. Methods The contents of PKC_α, PKC_βⅠ, PKC_βⅡ, PKC_γ, PKC_ε and PKC_ζ in mouse peritoneal macrophages were quantitatively determined by laser scanning confocal microscopy. The density of NF-κB positive cells in macrophages was detected by immunocytochemistry. Results The average fluorescence intensity of PKC_α and PKC_βⅡ in peritoneal macrophages of DNA injection group was significantly higher than that of the control group (P <0.01), while the average fluorescence intensity of PKC_βⅠ, PKC_γ, PKC_ε and PKC_ζ was not significantly different between the two groups Difference (P> 0.05). Bifidobacterium DNA injection group macrophages NF-kB positive cell density was significantly higher than the control group (P <0.01). Conclusion DNA of Bifidobacterium adolescentis activates macrophages by activating PKC_α, PKC_βII and NF-κB.