富氢液对大鼠肾缺血再灌注肾功能及TLR4/NF-κB信号通路表达的影响

• 作 者: 陈克研 董婉维
• 机 构: 中国医科大学实验动物部,辽宁沈阳,110122
• 出 处: 解剖科学进展
• 发表日期: 2017年2期
• 关键词: hydrogen rich saline renal ischemia-reperfusion TLR4 NF-κB

目的 探讨富氢液(HRS)对大鼠肾缺血再灌注肾功能及TLR4/NF-K B信号通路表达的影响.方法 SPF级雄性SD大鼠30只,随机分为假手术组(S组)、缺血再灌注组(I/R组)、富氢液组(HRS组),每组10只.S组麻醉开腹后右肾切除;I/R组行右肾切除,左肾缺血45min,再灌注60min,造肾缺血再灌注模型;HRS组于术前口服40 ml/kg富氢液,连续3d后建立I/R模型.每组于I/R后6h取取血样,分离血浆,多功能生化仪检测肌酐(Scr)和尿素氮(BUN)含量;ELISA检测IL-1β和TNF-α含量;另取肾脏,HE染色观察病理组织学变化,Western-blot检测TLR4、Myd88、NF-κB表达变化.结果 HRS可减轻由I/R造成的肾损伤,减低血浆中Scr、BUN、IL-1β和TNF-α含量,抑制肾脏TLR4、Myd88、NF-κB表达(P＜0.05).结论 HRS减轻由I/R引起的肾功能障碍,抑制炎性反应,对肾脏有一定的保护作用,其机制可能与TLR4/NF-KB信号通路有关.“,”Objective To explore the effect of hydrogen rich saline(HRS) on renal function and expression of TLR4/NF-kappa B signal pathway in rats with renal ischemia-reperfusion injury.Methods SPF SD rats(n=30) were randomly divided into sham group(S),ischemia reperfusion (I/R group) and HRS(40 ml/kg) group averagely.The serum creatinine(Scr) and urea nitrogen(BUN) contents were determined,contents of IL-1-α and TNF-α were detected by ELISA,kidney pathological changes were observed with HE staining,expression of Myd88,NF-kappa B and TLR4 determined by Western-blot.Results HRS reduced the renal damage caused by I/R,reduced the contents of Scr,BUN,IL-1 and TNF-α in plasma,inhibited the expressions of TLR4,Myd88 and NF-kappa B in kidney(P＜0.05).Conclusion The protective effect of HRS on the I/R kidney might be related to the TLR4/NF-κB signalpathway.