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以籼型超级杂交稻(Oryza sativaL.subsp.indica)恢复系9311的胚性愈伤组织为受体,采用改良的基因枪轰击法将构建在同一植物表达载体上的四价抗真菌病基因(RCH10,RAC22,β-Glu和B-RIP)导入到9311的基因组中.Southern印迹杂交结果表明,潮霉素抗性再生植株中,hpt标记基因和四价抗真菌病基因是连锁在一起并以孟德尔遗传方式进行传递的.部分转基因R1代和R2代植株分别在烟溪和三亚的典型稻瘟病鉴定圃中表现出对稻瘟病菌(Magnaporthe grisea(Hebert) Barr.)的高抗性.在高抗的转基因植株中,多个外源基因均能正常表达.将高抗稻瘟病的9311R2代转基因纯系与培矮64S原种进行杂交,杂种F1代除对稻瘟病仍可表现出高抗性外,还同时表现出对稻曲病和黑粉病明显提高的抗性.
The embryogenic callus of restorer line 9311 of indica super hybrid rice (Oryza sativa L. subsp. Indica) was used as the receptor. The modified gene gun bombardment method was used to transfer the tetravalent antifungal gene ( RCH10, RAC22, β-Glu and B-RIP) were introduced into the genome of 9311. Southern blot hybridization results showed that the hpt marker gene and the tetravalent antifungal gene were linked together in Mendelian inheritance.Several transgenic plants, R1 and R2, showed high resistance to Magnaporthe grisea (Hebert) Barr. In the typical rice blast identification nursery of Yanxi and Sanya, respectively High resistance to transgenic plants, a number of foreign genes can be normal expression of blast-resistant 9311R2 generation transgenic lines and Peiwa 64S original hybrids hybrid F1 generation in addition to the blast can still show high resistance In addition, it also showed a marked increase in resistance to smut and smut.