长时间低浓度暴露亚砷酸钠促进人正常膀胱上皮细胞增殖

• 作 者: 王晨 王菲 刘盛男 曹思奇 席淑华
• 机 构: 中国医科大学公共卫生学院,
• 出 处: 中国工业医学杂志
• 发表日期: 2015年03期
• 关键词: 正常膀胱 亚砷酸钠 SV-HUC-1细胞 四甲基 慢性砷暴露 细胞克隆 倒置荧光显微镜 传代培养 比色法 胰蛋白酶消化

以不同浓度亚砷酸钠(Na As O2)(0、0.1、0.5、1.0μmol/L)对人正常膀胱上皮细胞(SV-HUC-1)进行长期染毒,采用四甲基偶氮唑(MTT)比色法检测细胞活力。未经砷染毒的SV-HUC-1进行长期传代培养,细胞增殖活力没有发生任何改变;0.1、0.5μmol/L Na As O2处理20代后,细胞增殖活性显著增高,并呈剂量-反应增高趋势(r=0.632、P<0.01,r=0.939、P<0.01),而1.0μmol/L Na As O2处理的细胞其增殖活力未随染毒时间而发生变化(P>0.05)。提示长时间低浓度暴露Na As O2可以促进SV-HUC-1增殖。

The human normal bladder epithelial cells (SV-HUC-1) were exposed to different concentrations of sodium arsenite (0,0.1,0.5,1.0μmol / L) MTT) colorimetric assay cell viability. The proliferation of SV-HUC-1 without arsenic exposure did not show any change in cell proliferation. After treated with 0.1, 0.5μmol / L Na As O2 for 20 generations, the cell proliferation activity was significantly increased and the dose-response was increased (R = 0.632, P <0.01, r = 0.939, P <0.01). However, the proliferation activity of cells treated with 1.0 μmol / L Na As O2 did not change with the exposure time (P> 0.05). Suggesting that exposure of Na As O2 at low concentrations for a long time can promote the proliferation of SV-HUC-1.