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【目的】探讨川芎嗪对高氧致新生大鼠慢性支气管肺发育不良的影响。【方法】80只出生12 h内的清洁级Sprague-Dawley(SD)大鼠作为研究对象,随机分成4组(每组20只):A组为空气对照组;B组为空气+川芎嗪组;C组为高氧组;D组为高氧+川芎嗪组。B、D组新生鼠每日腹腔注射溶于生理盐水中的川芎嗪30 mg/kg,A、C组新生鼠每日腹腔注射等量生理盐水,持续14 d。第14 d每组随机选取10只新生鼠,处死取肺组织切片,HE染色法观察肺组织病理变化并计算辐射状肺泡计数(radical alveolar counts,RAC),应用分光光度计比色法检测各组大鼠肺组织丙二醛(ma-londialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)水平。应用ELISA方法测定肺组织中8-异前列腺素F2α(8-iso-PGF2α)含量。【结果】①C组显示明显的肺泡发育受阻,RAC值较A、B组均减少(P<0.05),但D组RAC较C组显著增加(P<0.05);②C组MDA水平、8-iso-PGF2α含量显著高于A、B组(P<0.05),D组MDA水平、8-iso-PGF2α含量较C组明显下降(P<0.05);③A、B、C组SOD比较无明显差异,D组使用丹参后其水平上升,与C组比较有统计学差异(P<0.05)。【结论】川芎嗪对高氧所致的支气管肺发育不良有保护作用,其机制可能是通过抗氧自由基、减轻肺部炎性反应而发挥作用。
【Objective】 To investigate the effect of tetramethylpyrazine on chronic bronchopulmonary dysplasia in neonatal rats with hyperoxia. 【Methods】 Eighty Sprague-Dawley (SD) rats of clean grade within 12 hours of birth were randomly divided into 4 groups (20 in each group): Group A was air control group; Group B was air plus tetramethylpyrazine group. Group C was hyperoxia group; group D was hyperoxia + ligustrazine group. Rats in Groups B and D were injected intraperitoneally with Ligustrazine (30 mg/kg) dissolved in physiological saline daily. Rats in groups A and C were injected intraperitoneally with normal saline for 14 days. On the 14th day, 10 newborn rats in each group were randomly selected, and lung tissues were taken for sacrifice. HE staining was used to observe the pathological changes of lung tissue and calculate radial alveolar counts (RAC). Spectrophotometer colorimetry was used to detect each group. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels in rat lung tissue. The content of 8-iso-prostaglandin F2α (8-iso-PGF2α) in lung tissue was determined by ELISA. 【Results】 1C group showed obvious obstructed alveolar development, RAC values were lower than those in group A and B (P<0.05), but RAC in group D was significantly higher than that in group C (P<0.05); 2C group MDA level, 8-iso The level of PGF2α was significantly higher than that of group A and B (P<0.05). The levels of MDA and 8-iso-PGF2α in group D were significantly lower than those in group C (P<0.05). There was no significant difference in SOD between groups 3A, B, and C. The use of salvia miltiorrhiza in group D increased its level, compared with group C, there was a statistically significant difference (P<0.05). 【Conclusion】 Tetramethylpyrazine has a protective effect against hyperoxia-induced bronchopulmonary dysplasia, and its mechanism may be through antioxidant free radicals and reduction of pulmonary inflammatory responses.