Preparation of Anti—Cardiac Troponin I Monoclonal Antibodies and Their Characterization with Surface

• 作 者: WEIJing-yan LIUXia SONGDa-qian
• 机 构: KeyLaboratoryofMolcularEnzymologyandEngineeringofministryofEducation,CollegeofChemistry,ThethirdClin
• 出 处: 高等学校化学研究：英文版
• 发表日期: 2003年2期
• 关键词: 强心剂 肌钙蛋白Ⅰ 单克隆抗体 生物传感器 细胞 胞质基因 Troponin I Monoclonal antibodies Characterizatio
• 基金项目: 国家自然科学基金，the Health Department of Jilin Province

Cardiac troponin I(cTnI) was separated and purified from human left ventricular tissue by affinity chromatographic method and used to immunize Balb/c mice by intraperitoneal injection and four hybridoma cell lines, which secreted monoclonal antibody(mAb)