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我们用培养的大鼠胚胎中脑黑质多巴胺能神经元,移植于帕金森氏病(简称PD)大鼠模型脑内,可使损伤黑质所引起的异常行为得到纠正。在此基础上,试图建立培养的人胚中脑多巴胺能神经元库,可为PD患者随时提供移植用的神经元。特选用体外培养法,将吸刮流产或经水囊引产的中期人胎(2～4月)共10例,切成小块组织和分离细胞悬液培养。结果表明,其生长发育过程和大鼠胚胎中脑的相似。但是人胎个体差异较大,培养时间最长的达7周。水囊引产的比吸刮流产的成活时间长。两种培养方法的所见基本相同。培养4小时细胞全部贴壁,少数细胞已有突起。第2天起,见组织块中首先爬出成纤维细胞和胶质细胞,继而神经元沿胶
We use the cultured rat embryonic mesencephalic substantia nigra dopaminergic neurons transplanted into the brain of Parkinson’s disease (PD) rat model to correct the abnormal behavior caused by the injury of substantia nigra. On this basis, trying to establish a culture of human embryonic mesencephalic dopaminergic neuron library, for PD patients at any time to provide transplantation of neurons. Specially selected in vitro culture method, abortion abortion or abortion of the mid-term human fetus (2 to 4 months) a total of 10 cases, cut into small pieces of tissue culture and cell suspension culture. The results showed that the process of growth and development and rat embryonic midbrain similar. However, individual differences in human fetuses larger, the longest incubation time up to 7 weeks. Induction of hydatid sucking than abortion survival time. The two methods of culture are basically the same. Cells cultured for 4 hours all adherent, a small number of cells have protrusions. On the second day, see the first out of the tissue block fibroblasts and glial cells, and neurons along the glue