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本实验应用聚丙烯酰胺凝胶盘状电泳方法对莫桑比克罗非鱼(Tilapia mossambica)和尼罗罗非鱼(Tilapia milotica)及其杂交种——福寿鱼(T.missambica ?×T.nilotica ?)的血清蛋白进行了分离,同时对其雌性特异蛋白进行了氨基酸组成分析。莫桑比克罗非鱼的血清蛋白电泳图型具有 12—14条蛋白质带,尼罗罗非鱼有12—13条蛋白质带,杂交种—福寿鱼有12—15条蛋白质带;三种鱼血清蛋白电泳图型在雌、雄性别上有明显的差异。莫桑比克罗非鱼和尼罗罗非鱼的成熟雌鱼有两条新的蛋白质带,这些带都比同种的雄鱼所显示的颜色深。杂交种——福寿鱼的血清蛋白电泳图型,为其亲本的中介型。 此外,本文还对雌性特异蛋白的蛋白质带,进行了氨基酸组成分析。
In this study, polyacrylamide gel electrophoresis was used to determine the genetic diversity of Tilapia mossambica and Tilapia milotica and their hybrid T.missambica × T. nilotica Of serum proteins were separated, at the same time its female specific protein amino acid composition analysis. The serum protein electrophoresis pattern of tilapias in Mozambique has 12-14 protein bands, 12-13 protein bands in Nile tilapia, 12-15 protein bands in hybrids - longevity fish, and 3 fish serum protein electrophoresis patterns There are obvious differences between female and male. Mature female tilapia and Nile tilapia in Mozambique have two new protein bands, all of which are darker than those shown by the same species of males. Hybrids - Fukushima serum protein electrophoresis patterns, the parents of their intermediary. In addition, the amino acid composition analysis of the protein bands of female specific proteins was also carried out.