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目的:探讨硒蛋氨酸对食管癌细胞系EC9706环氧合酶-2(COX-2)表达的影响。方法:采用MTT比色法、细胞生长曲线描绘观察硒蛋氨酸对食管癌细胞系EC9706增殖的影响,琼脂糖凝胶电泳法检测DNA ladder,细胞免疫化学方法检测EC9706COX-2的表达。结果:硒蛋氨酸呈时间、剂量依赖性方式抑制EC9706细胞增殖,抑制食管癌细胞系EC9706COX-2的表达,诱导细胞凋亡。结论:硒蛋氨酸可能通过抑制食管癌细胞系EC9706COX-2的表达从而抑制EC9706细胞增殖,诱导细胞凋亡。
Objective: To investigate the effect of selenomethionine on the expression of cyclooxygenase-2 (COX-2) in esophageal carcinoma cell line EC9706. Methods: The effect of selenomethionine on the proliferation of esophageal carcinoma cell line EC9706 was observed by MTT assay and cell growth curve. DNA ladder was detected by agarose gel electrophoresis and the expression of EC9706 COX-2 was detected by immunocytochemistry. Results: Selenomethionine inhibited the proliferation of EC9706 cells in a time-and dose-dependent manner, and inhibited the expression of EC9706 COX-2 and induced the apoptosis of EC9706 cells. Conclusion: Selenomethionine may inhibit EC9706 cell proliferation and induce apoptosis by inhibiting the expression of esophageal cancer cell line EC9706COX-2.