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Objective: To observe the expression of N-acetyl-glucosamine-6-O-sulfotransferase (GN-6-ST)in the endometrium during the window stage of implantation from infertile women before IVF-ET treatment, we compared the GN-6-ST gene expression level between the women with succeeded and failed implantation, and investigated the roles of selectin and its ligands in the embryo implantation.Methods: The hysteroscopy and endometrial biopsies were performed in patients prior to undergoing IVF-ET treatment in the IVF Center of the First Affiliated Hospital of Nanjing Medical University from July 2004 to March 2005.Fourteen patients who succeeded in implantation were taken as study group, while the 28 infertile patients with failed implantation served as control group.The RT-PCR method was used to detect the mRNA levels of N-ac-etyl-glucosamine-6-O-sulfotransferase in the endometrium during the window stage of imp-lantation of the women from both groups.Results: For these infertile patients with succeeded implantation, the average mRNA expression level of acetylglucosamine-6-O-sulfotransferase in the endometrium during the window stage of implantation was (0.65±0.33),while for those with failed implantation cycle, the average mRNA expression level was (0.41±0.36), which was significantly lower than that of study group, P＜0.05.Conclusions: The combination of the selectin and ligands may play a role in the embryo implantation capacibility.