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目的:通过实时观察一次性力竭运动过程中大鼠纹状体葡萄糖和乳酸浓度的动态变化规律,揭示运动性中枢疲劳形成过程中脑能量代谢的特征。方法:8周龄雄性Wistar大鼠20只分为两组,纹状体葡萄糖、乳酸测定组(第1组)和外周血葡萄糖、乳酸测定组(第2组),每组10只。采用微透析-电化学联用的活体检测技术,实时监测大鼠(第1组)在一次性力竭运动过程中纹状体细胞外液中葡萄糖和乳酸的代谢变化,并从尾静脉采血动态监测大鼠(第2组)外周血液中葡萄糖和乳酸浓度的变化。结果:(1)与安静状态相比,运动初期大鼠纹状体胞外乳酸浓度显著升高(P<0.05),运动后期直至恢复期均显著降低(P<0.05,P<0.01);而胞外葡萄糖浓度在运动初期无明显变化,在运动后期开始下降,甚至在恢复期的90分钟内仍显著低于安静水平(P<0.05,P<0.01)。(2)大鼠外周血糖浓度随着运动时间的延长而显著降低,在运动力竭以及恢复期血糖水平均显著低于安静水平(P<0.05,P<0.01);大鼠血乳酸浓度在力竭运动过程中显著高于安静时水平(P<0.05),而在运动结束后即迅速恢复至安静时水平。结论:力竭运动过程中,持续的外周低血糖导致脑对于葡萄糖摄取不足,出现脑葡萄糖和乳酸浓度降低,中枢能量物质葡萄糖和乳酸代谢的显著降低可能是产生运动性中枢疲劳的一个重要的神经生物学机制。
OBJECTIVE: To observe the dynamic changes of glucose and lactate concentrations in rat striatum during a one-time exhaustive exercise to reveal the characteristics of brain energy metabolism in the process of exercise-induced central fatigue. Methods: Twenty male Wistar rats aged 8 weeks were divided into two groups: striatal glucose, lactate assay group (group 1), peripheral blood glucose and lactate assay group (group 2). Microdialysis - electrochemical biochemical detection of live, real-time monitoring of rats (group 1) during a one-time exhaustive exercise of striatal extracellular fluid glucose and lactate metabolism, and from the tail vein blood collection dynamic Changes of glucose and lactate concentrations in the peripheral blood of rats (group 2) were monitored. Results: (1) Compared with resting state, the concentration of extracellular lactate in striatum significantly increased (P <0.05) and decreased from late period to convalescent stage (P <0.05, P <0.01) The extracellular glucose concentration did not change significantly in the early period of exercise and began to decrease in the late period of exercise. It was still significantly lower than the resting level within 90 minutes of recovery (P <0.05, P <0.01). (2) The concentration of peripheral blood glucose in rats decreased significantly with the prolongation of exercise time, the level of blood glucose in exercise exhaustion and convalescent phase were significantly lower than those in resting state (P <0.05, P <0.01) During exhaustive exercise, they were significantly higher than those at rest (P <0.05), but rapidly recovered to quiescence after exercise. CONCLUSIONS: Persistent peripheral hypoglycaemia in the exhaustive exercise leads to inadequate brain glucose uptake, decreased brain glucose and lactate concentrations, and a significant decrease in central energy metabolites glucose and lactate metabolism that may be an important nerve that produces motor central fatigue Biological mechanism.