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[目的]检测溃疡性结肠炎患者血清低氧诱导因子-1α(HIF-1α)的含量,探讨其在溃疡性结肠炎发病机制中的调控作用及其与患者病情活动性和严重性的关系。[方法]采用酶联免疫吸附试验(ELISA)定量检测13例缓解期(缓解期组)、47例活动期(活动期组)UC患者及10例健康体检者(正常对照组)血清中HIF-1α含量,并对活动期组患者行肠镜检查,评价病情轻重程度。[结果]活动期组HIF-1α含量[(73.21±28.65)ng/L]显著高于缓解期组[(44.54±14.75)ng/L]与正常对照组[(42.83±15.49)ng/L],P<0.05;缓解期组与正常对照组比较差异无统计学意义(P>0.05)。HIF-1α与病情活动性、病情分型及内镜表现分级呈正相关。[结论]HIF-1α作为一种转录因子可能在UC发病机制中充当重要角色,并可能作为评价UC患者病情活动性及严重程度的良好指标。
[Objective] To detect the serum level of hypoxia-inducible factor-1α (HIF-1α) in patients with ulcerative colitis, and to explore its regulatory role in the pathogenesis of ulcerative colitis and its relationship with disease activity and severity. [Methods] Serum levels of HIF-1α in 13 remission (remission stage), 47 active stage (active stage) UC patients and 10 healthy control subjects (normal control group) were quantitatively determined by enzyme linked immunosorbent assay (ELISA) 1α content, and active group patients underwent colonoscopy to assess the severity of the disease. [Results] The level of HIF-1α in active group [(73.21 ± 28.65) ng / L] was significantly higher than that in remission group [(44.54 ± 14.75) ng / L] and [42.83 ± 15.49] ng / L in normal control group , P <0.05; There was no significant difference between remission group and normal control group (P> 0.05). HIF-1α and disease activity, disease classification and endoscopic performance was positively correlated. [Conclusion] As a transcription factor, HIF-1α may play an important role in the pathogenesis of UC and may be used as a good indicator to evaluate the activity and severity of UC patients.