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DNA分析是一种有效地鉴别木材的方法,但它要求要从木材中得到足够质量和数量的DNA。因此,本试验以降香黄檀气干材为原料,采用改良的CTAB法、QIAGEN试剂盒法和PTB法分别提取降香黄檀心材和边材部位的DNA,比较从不同部位木材组织中提取DNA的质量差异,以期为从心材和边材组织中提取DNA探寻合适的方法。结果表明,3种方法从边材和心材部位提取DNA浓度范围分别为:75.95～937.38 ng·μL-1,4.46～806.56ng·μL-1,其中PTB法从边材和心材部位提取的DNA浓度都是最高的,试剂盒法提取的DNA浓度都是最低的。3种方法提取的边材部位DNA经纯化处理后能够满足PCR扩增目的片段的要求;只有PTB法提取的心材部位的DNA经纯化处理后能够满足PCR扩增目的片段的要求。3种方法都能够从边材组织中提取出DNA,PTB法更适合从心材组织中提取DNA。
DNA analysis is a method of identifying wood efficiently, but it requires getting enough quality and quantity of DNA from the wood. Therefore, in this experiment, the desiccated sandalwood sapwood was used as raw material, and the DNA of the descended rosewood heartwood and sapwood were extracted by modified CTAB method, QIAGEN kit method and PTB method, respectively. DNA from different parts of the wood tissue was extracted In order to explore suitable methods for extracting DNA from heartwood and sapwood tissues. The results showed that the DNA concentration ranged from 75.95 to 937.38 ng · μL-1,4.46 to 806.56 ng · μL-1 for the three methods, respectively. The DNA concentration extracted from the sapwood and heartwood by PTB method Are the highest, kit DNA extraction method is the lowest concentration. The purified DNA from the sapwood of the three methods can meet the requirement of PCR amplification. Only the DNA of the heartwood extracted by PTB can be purified to meet the requirement of PCR amplification. All three methods are capable of extracting DNA from sapwood tissue, and the PTB method is more suitable for extracting DNA from heartwood tissue.