产(R)-α-基苯乙酸的芽抱杆菌B26的鉴定与诱变育种(英文)

• 作 者: 陈秉梅 许小平 侯志国 李忠琴 阮文兵
• 机 构: College of Chemistry and Chemical Engineering,Fuzhou University,College of Fisheries,Jimei Universit
• 出 处: Chinese Journal of Chemical Engineering
• 发表日期: 2011年04期
• 关键词: 芽抱杆菌 苯乙酸 fermentation 诱变育种 agitation sequencing viable clone bacterium irradiati

A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.

A bacterium strain B26 capable of producing (R) -α-hydroxyphenylacetic acid [(R) -HPA] (yield, 47.5%; enantiomeric excess, 99.1%) from phenylglyoxylic acid (PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA (ribosomal DNA) sequencing. Phylogenic analysis showed that the strain was most similar to Bacillus sp. enrichment culture clone clone SYW5 (FJ601635.1) and Bacillus cereus strain FM-4 (EU794727.1). Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis. Atmospheric viable mutants, B. sp. UV-38 and B. sp. ULi-11 exemplary better productivities than the wild type. Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11 (yield, 65.4 %) compared to that with B.sp.B26 (yield, 47.0%) when cultured in fermentation broth (pH 7.2) at 32 ° C with an agitation speed of 180 r · min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.