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目的 研究建立一种肝癌特异 GGT 快速测定方法用于肝癌的早期诊断或作为其它肝癌标志物的补充。方法 利用免疫学和生物化学方法将肝癌特异 GGT 抗体吸附并固定到醋酸纤维薄膜上 ,对患者血清中 GGT 进行定性检测。结果 本法对原发性肝癌 (PHC)诊断敏感性为 84.82 % ,特异性为 96.4% ,对继发性肝癌 (MHC)诊断阳性率为 45.78% ;本法对 AFP阴性的 PHC病人检测阳性率可达 60 .61 % ,可有效补充 AFP阳性率较低的不足 ;本法检测不需要贵重仪器设备 ,仅需指端采血即可进行 ,1 0分钟出结果。结论 本法简便、快速、微量、灵敏、经济 ,可用于 PHC的诊断 ,鉴别诊断以及 PHC的早期发现 ,也可作为其它肝癌标志物的补充在临床应用。
Objective To establish a rapid and specific GGT assay for liver cancer for the early diagnosis of liver cancer or to supplement other liver cancer markers. Methods Immunohistochemistry and biochemical methods were used to adsorb and immobilize hepatoma specific GGT antibody on the acetate membrane. The serum GGT was qualitatively detected. Results The sensitivity and specificity of this method for the diagnosis of primary liver cancer (PHC) were 84.82% and 96.4%, respectively. The positive rate of this method was 45.78% for the diagnosis of secondary liver cancer (MHC). The positive rate of this method for detecting AFP-negative PHC patients Up to 60.61%, which can effectively supplement the lack of AFP-positive rate; this method does not require expensive instruments and equipment testing, just finger-side blood can be carried out, 10 minutes out of the results. Conclusion The method is simple, rapid, sensitive, and economical. It can be used in the diagnosis, differential diagnosis of PHC and the early detection of PHC. It can also be used as a supplement of other liver cancer markers in clinical application.