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目的:探讨新疆主要民族之间食管癌患者血清蛋白质指纹图谱的差异.方法:用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)检测43例汉族、43例维吾尔族及41例哈萨克族食管癌血清蛋白质指纹图谱,所得结果用ZUCI-PDAS软件分析处理,筛选血清差异性蛋白质.结果:同民族间的血清蛋白质指纹图谱存在明显差异.汉族组与维吾尔族组中质荷比为4310.0109,8713.0142,7993.0223的3个蛋白质峰差异具有统计学意义(P<0.05);汉族组与哈萨克族食管癌组中质荷比为4310.0184,8167.9277,8158.1117,13789.4864,8067.7056,4611.9098,7993.4422,16146.8706的8个蛋白质峰差异具有统计学意义(P<0.05);维吾尔族组与哈萨克族组中质荷比分别为9161.7944,4611.6342,6649.6163,4979.3807的4个蛋白质峰差异具有统计学意义(P<0.05);质荷比4310.0109的蛋白峰在汉族组中呈低表达,而在维吾尔族组和哈萨克族组中均呈高表达.在维吾尔族组和哈萨克族组之间该蛋白峰未见到有差异.结论:汉族、维吾尔族及哈萨克族食管癌患者之间血清蛋白质指纹图谱的差异具有统计学意义,在新疆地区建立不同民族食管癌患者血清蛋白质指纹图谱疾病诊断模型有一定的临床意义.
Objective: To explore the differences of serum protein fingerprints between esophageal cancer patients in Xinjiang ethnic groups.Methods: 43 cases of Han, 43 Uighurs and 41 Kazakh were detected by SELDI-TOF-MS, Serum protein fingerprints of esophageal cancer were analyzed by ZUCI-PDAS software.Results: There was a significant difference in serum protein fingerprinting between different ethnic groups.The mass-to-charge ratio in Han and Uygur ethnic groups was 4310.0109 , 8713.0142 and 7993.0223, respectively (P <0.05). In Han and Kazakh esophageal carcinomas, the mass-to-charge ratios of 4310.0184, 8167.9277, 8158.1117, 13789.4864, 8067.7056, 4611.9098, 7993.4422 and 16146.8706 (P <0.05). There were significant differences in the four protein peaks between the Uygur and Kazakh groups with mass / charge ratios of 9161.7944, 46611.6342, 6649.6163 and 4979.3807 (P <0.05), respectively. The protein peak with a mass-to-charge ratio of 4310.0109 was low expressed in the Han population and highly expressed in the Uygur population and the Kazakh population. In the Uygur group There was no difference between the Kazakh group and the protein peak.Conclusion: The differences of serum protein fingerprints between Han, Uygur and Kazak esophageal cancer patients were statistically significant, and the serum proteins of different ethnic esophageal cancer patients were established in Xinjiang Fingerprint disease diagnosis model has some clinical significance.