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目的 建立青海藏族人群T淋巴细胞免疫表型参考值范围,阐明棘球蚴感染与宿主之间相互作用的机制.方法 采集确诊的青海省藏族泡型包虫病患者和健康成人外周血各50份,囊型包虫病患者外周血42份,采用流式细胞仪检测T淋巴细胞亚群CD3+、CD4+、CD8+百分率以及表达IL-4、IFN-γ的CD19+和CD56+、CD25+和HLA-DR+以及CD4+、CD8+细胞百分率,分析藏族人群感染细粒棘球蚴和泡球蚴后机体免疫系统T淋巴细胞亚群的变化. 结果 青海省藏族16～60岁健康成人外周血淋巴细胞亚群参考值范嗣分别为:①总T细胞(61.33±7.78)％;②CD3+/CD4+细胞(35.47±6.22)％;③CD++/CD8+细胞(31.12±6.44)％;④CD4+ /CD8+比值1.21±0.40.⑤B细胞CD3/CD19+ (12.19±4.77)％;⑥NK细胞CD3-/CD56+(19.89±7.45)％;⑦CD25+细胞(7.43±5.58)％;⑧HLA DR+细胞(34.42±12.86)％.两型包虫病患者总T细胞水平均高于健康人群(P＜0.05),CE患者CD25+细胞和AE患者CD3/CD19+低于健康人群(P＜0.05),CE患者的CD25+细胞、IL-4/CD4+水平低于AE患者(P＜0.05);CE患者CD3+/CD4+细胞水平男性低于女性(P＜0.05),IL-4/CD8+水平男性高于女性(P＜0.05),AE患者总T细胞水平男性高于女性(P＜0.05). 结论 建立了青海省藏族人群淋巴细胞免疫表型参考值范嗣,并证实包虫病患者血液淋巴细胞亚群发生改变,一定程度上影响了机体抵抗包虫感染的免疫保护作用.“,”Objective To determine reference values for T lymphocytes in the Tibetan population in Qinghai in order to further clarify the mechanism of interaction between echinococcosis and the host.Methods Peripheral blood samples were collected from 50 patients with alveolar echinococcosis,50 healthy Tibetan adults in Qinghai,and 42patients with cystic echinococcosis.All of the samples were subjected to flow cytometry (FCM).The proportion of T lymphocyte subsets CD3+,CD4+,and CD8+,the proportion of T lymphocyte subsets CD19+ (B cells) and CD56+ (NK cells),the proportion of CD25 + cells,the proportion of HLA-DR+ cells,IL-4 and IFN-γ levels,and the ratio of CD4+ and CD8+ cells were examined to analyze changes in T lymphocyte subsets in the immune system of Tibetans after infection with Echinococcus granulosus or development of alveolar echinococcosis.Results The reference range for peripheral blood lym phocyte subsets in healthy Tibetan adults ages 20-60 in Qinghai was:①total T cells:61.33 ±7.78％;②CD3+/CD4 + cells:35.47±6.22％;③CD++/CD8+ cells:31.12±6.44％;④CD4+/CD8+ ratio:1.21±0.40;⑤Bcells:12.19± 4.77％;⑥NK cells:19.89±7.45％;⑦CD25+ cells:7.43 ± 5.58％;and ⑧HLA-DR+ cells:34.42 ± 12.86％.Patients with cystic or alveolar echinococcosis had a higher proportion of total T cells than healthy people had (P＜0.05).Patients with cystic echinococcosis had a lower proportion of CD25+ cells and patients with alveolar echinococcosis had a lower proportion of CD3 /CD19+ cells than healthy people had (P＜0.05).Patients with cystic echinococcosis had a lower proportion of CD25 + cells and IL-4/CD4+ cells than patients with alveolar echinococcosis had (P＜0.05).Male patients with cystic echinococcosis had a higher proportion of CD3+/CD4 cells than female patients had (P＜0.05).Male patients had a higher proportion of IL 4/CD8+ cells than female patients had (P＜0.05).Male patients with alveolar echinococcosis had a higher proportion of total T cells than female patients had (P＜0.05).Conclusion The reference range for lymphocyte immunophenotypes was determined in the Tibetan population in Qinghai Province.The proportion of lymphocyte subsets in patients with echinococcosis changed,influencing immune mechanisms and parasite growth in the host to an extent.