论文部分内容阅读
目的 :根据甘露糖结合蛋白 (MBP)基因多态性对其功能的影响 ,探讨MBP基因多态性与IgA肾病免疫病理间的联系。 方法 :选取 1 4 7例IgA肾病 (IgAN)患者 ,其中 77例为肾小球单纯IgA伴C3沉积 ,70例为肾小球IgA ,IgG ,IgM伴C3,C1q沉积。使用PCR RFLP方法对MBP基因多态性与IgAN免疫沉积的关系进行了观察。 结果 :①本研究中正常人群MBP基因多态性分布与文献报道的华人及高加索人种接近并无显著差异。②IgAN中大量免疫沉积 (IgA ,IgG ,IgM ,C3,C1q)患者MBP基因GGC/GAC基因型的发生频率明显高于单纯IgA沉积 (IgA ,C3)患者 (41 4 %vs 1 9 5 % ,P <0 0 1 ) ,而GGC/GGC基因型的发生频率明显低于单纯IgA沉积患者 (5 5 7%vs 76 6 % ,P<0 0 1 )。IgAN的大量免疫沉积患者GAC等位基因的发生频率明显高于单纯IgA沉积患者 (0 2 36vs 0 1 36 ,P <0 0 5 ) ,而GGC等位基因的发生频率明显低于单纯IgA沉积患者 (0 76 4vs0 86 4,P <0 0 5 ) ,GAC等位基因与IgAN的大量免疫沉积明显相关 (OR =1 95 ,95 %CI:1 0 6～ 3 5 8) 结论 :IgAN免疫病理表现多样性受基因背景的影响 ,MBP变异型等位基因与IgAN的大量免疫沉积相关
OBJECTIVE: To investigate the relationship between MBP gene polymorphism and immunopathology of IgA nephropathy according to the effect of MBP polymorphism on its function. Methods: One hundred and seventy-seven patients with IgA nephropathy (IgA) were enrolled. Among them, 77 were glomerular simple IgA with C3 deposition and 70 were glomerular IgA, IgG, IgM with C3 and C1q deposition. The relationship between MBP gene polymorphism and IgAN immunoprecipitation was observed by PCR RFLP. Results: ① The distribution of MBP polymorphism in normal population in this study was not significantly different from that of Chinese and Caucasians reported in the literature. ② The frequency of GGC / GAC genotypes of MBP gene was significantly higher in IGAN patients with IgA, IgG, IgM, C3, C1q than those with IgA, C3 (41.4% vs 195%, P <0 01). However, the frequency of GGC / GGC genotype was significantly lower than that of IgA alone (57 57% vs 76 6%, P 0 01). The frequency of GAC alleles in IgAN immunosuppressive patients was significantly higher than that in IgA -positive patients (0 2 36 vs 0 1 36, P 0 05), but the frequency of GGC alleles was significantly lower than that in patients with IgA alone (0 76 4vs0 86 4, P 0 05). The GAC allele was significantly associated with IgA immunoprecipitation (OR = 95 95% CI: 106 ~ 358). Conclusion: Immunohistochemical results of IgAN Diversity is influenced by genetic background and the MBP variant allele is associated with extensive immunoprecipitation of IgAN