论文部分内容阅读
目的:建立临床有效方剂双莲方的全时段多波长融合指纹图谱,为双莲方的质量控制提供依据。方法:采用高效液相色谱法,Welch Ultimate LP-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相0.1%甲酸水(A)-甲醇(B)进行梯度洗脱(0~20 min,25%~60%A;20~40 min,60%~65%A%;40~70 min,65%~45%A),流速1.0 m L·min~(-1),柱温30℃,检测波长254,300,320,365 nm。结果:以木犀草素的色谱峰为参照峰,共确定18个色谱峰,建立了临床有效方剂双莲方的全时段多波长指纹图谱。根据此图谱,对其他成分进行相对定量,规定双莲方中其他成分相对木犀草素的含量不得低于0.39,0.24,0.46,0.45,0.73,0.28,0.16,0.41,0.51,2.37,0.77,0.22,0.60,0.36,0.62,0.80,0.52,0.96。结论:该方法简单、准确、科学,适用于双莲方的质量评价。
Objective: To establish full-time multi-wavelength fusion fingerprints of Shuanglianfang, a clinically effective prescription, to provide the basis for the quality control of Shuanglianfang. Methods: The gradient elution was carried out on a Welch Ultimate LP-C_ (18) column (4.6 mm × 250 mm, 5 μm) with a mobile phase of 0.1% formic acid in water (A) 20 min, 25% -60% A, 20-40 min, 60% -65% A%, 40-70 min, 65% -45% A at a flow rate of 1.0 m L · min -1. 30 ℃, detection wavelength 254,300,320,365 nm. Results: The chromatographic peak of luteolin was taken as the reference peak, a total of 18 peaks were identified, and the full-time multi-wavelength fingerprint of the clinical effective prescription Shuanglianfang was established. According to this map, the relative quantification of other components, the provisions of other components of Shuanglian Fang relative luteolin content of not less than 0.39,0.24,0.46,0.45,0.73,0.28,0.16,0.41,0.51,2.37,0.77,0.22 , 0.60,0.36,0.62,0.80,0.52,0.96. Conclusion: The method is simple, accurate, scientific and suitable for quality evaluation of Shuanglianfang.