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目的:研究临床胶质瘤标本中激活转录因子5(ATF5)启动子区CpG岛甲基化状态及临床意义。创新点:首次发现在胶质瘤标本中ATF5的甲基化水平下调,其表达水平下调。方法:收集35临床胶质瘤组织及5例急性脑外伤组织作为对照,应用亚硫酸盐测序技术检测ATF5的甲基化水平,并结合临床病理资料进行分析;实时荧光定量聚合酶链式反应(qRT-PCR)检测所有标本中ATF5 mRNA的表达水平变化。结论:5例正常脑组织、10例低级别胶质瘤及25例高级别胶质瘤的甲基化比例分别为87.78%、73.89%和47.70%(图2),两组相比差异有统计学意义(P<0.05;图3a和3b);qRT-PCR结果表明,与对照相比,胶质瘤标本中ATF5表达水平上升(P<0.05;图3d)。综上所述,胶质瘤组织中ATF5基因启动子区CpG岛的甲基化状态对该基因的表达有重要意义。
Objective: To investigate the methylation status of CpG island in promoter region of activated transcription factor 5 (ATF5) in clinical glioma specimens and its clinical significance. Innovative point: For the first time, it was found that the methylation level of ATF5 was down-regulated in glioma specimens and its expression level was down-regulated. Methods: 35 clinical glioma tissues and 5 acute brain injury tissues were collected as control. The methylation level of ATF5 was detected by sulfite sequencing and analyzed with clinicopathological data. Real-time fluorescence quantitative polymerase chain reaction qRT-PCR) was used to detect the expression of ATF5 mRNA in all samples. CONCLUSION: The methylation rates of 5 normal brain tissues, 10 low-grade gliomas and 25 high-grade gliomas were 87.78%, 73.89% and 47.70%, respectively (Figure 2). There were statistically significant differences between the two groups (P <0.05; Fig. 3a and 3b); qRT-PCR results showed that the expression of ATF5 in glioma specimens increased compared with the control (P <0.05; Fig. 3d). In summary, the methylation status of the CpG island in the promoter region of ATF5 gene in glioma tissue is of great significance to the gene expression.