论文部分内容阅读
Aim: The aim of our study was to develop an effective gone delivery system for ovarian cancer gone therapy. Methods: The expression of heparin sulfate proteoglycan (HSPG) and integrins αvβ3 and αvβ5 were analyzed with flow cytometry on 2 human ovarian cancer cell lines (OVCAR-3 and SKOV-3ip). The gone transduction efficiencies were evaluated with recombinant adeno-associated viral vector (rAAV)2-green fluorescent protein or rAAV2-1actase Z followed by flow eytometry or cytohistochemistry staining. The effect of 17β-estradiol on ovarian cancer cell proliferation, HSPG, the expressions of integrins αvβ3 and αvβ5, and adeno-associated viral vector (AAV)2-mediated gone transduction were determined. Results: In the present study, we found: (1) a variation in HSPG and the expressions of integrins αvβ3 and αvβ5 between OVCAR-3 and SKOV-3ip; (2) that 1713-estradiol was shown to significantly stimulate cell proliferation and integrin β5 expression in certain ovarian cancer cell lines; and (3) integrin-targeted A520/N584RGD-rAAV2, which has alteative interactivity with integrins and abrogates the binding capacity HSPG, showed much higher gone transduction efficiency in ovarian cancer cells than rAAV2 in the presence/ absence of 17β-estradiol. Moreover, this RGD-modified rAAV2 exerted more efficient transduction in ovarian cancer cells in response to 17β-estradiol. Conclusion: Our findings implied that A520/N584RGD-rAAV2 may offer great potential for ovarian cancer treatment in vivo.