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目的探讨反义寡核苷酸（ＡＳＯＮ）特异性抗病毒作用。方法在ＨｅｐＧ２２．２．１５细胞中观察了４段１６聚针对乙型肝炎病毒（ＨＢＶ）基因不同功能区的ＡＳＯＮ对病毒复制的抑制作用。结果ＡＳＯＮ能显著抑制ＨＢＶ基因的抗原表达（Ｐ＜０．００１），在Ｓ基因区设计的ＡＳＯＮ对ＨＢｓＡｇ的抑制作用明显优于Ｃ基因区的ＡＳＯＮ（Ｐ＜０．０５）。反之，在Ｃ基因区设计的ＡＳＯＮ对ＨＢｅＡｇ的抑制作用又明显优于Ｓ和Ｐｒｅ－Ｓ２基因区的ＡＳＯＮ（Ｐ＜０．０１）。４段ＡＳＯＮ的联合用药并不能增强其对ＨＢＶ的抑制作用。结论ＡＳＯＮ可能成为一种有开发潜力的抗病毒药物。
Objective To investigate antisense oligonucleotide (ASON) specific antiviral effect. Methods In HepG22.2.15 cells, we observed the inhibitory effect of ASON on viral replication in four segments of 16-poly-AS against different functional regions of hepatitis B virus (HBV) gene. Results ASON could significantly inhibit the expression of HBV antigens (P <0.001). The inhibitory effect of ASON on S gene was better than that of C genes (P <0.05). Conversely, the inhibitory effect of ASON designed in the C gene region on HBeAg was significantly better than that of ASON in the S and Pre-S2 gene regions (P <0.01). 4-ASON combination does not enhance its inhibitory effect on HBV. Conclusion ASON may become a development potential of antiviral drugs.