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眼球结构精细,各部分组织的软硬程度也比较悬殊,所以制作全眼球切片标本历来都是采用火棉胶切片法,但该法切片较厚、制作费时,不适宜病理活检诊断。笔者进行了方法学改进,采用石蜡切片法,质量可以满足病理诊断要求。现介绍于下: 一、方法 1.新鲜眼球直接投入固定液(甲醛20ml,95%酒精75ml,冰醋酸5ml),同时用注射器抽吸固定液3～5ml,选定一处眼肌起始点旁巩膜部刺入,接着注入固定液直至眼球塌陷处鼓起为止。如眼压高、眼
The structure of the eyeballs is fine, and the degree of softness and hardness of each part of the tissue are also very different. Therefore, the production of whole eyeball specimens has historically used the collodion method, but the method is relatively thick and time-consuming, and is not suitable for pathological biopsy diagnosis. The author has improved the methodology and adopted the paraffin section method. The quality can meet the requirements of pathological diagnosis. Now introduced in the following: First, the method 1. The fresh eye directly into the fixative (formaldehyde 20ml, 95% alcohol 75ml, glacial acetic acid 5ml), while the syringe suction fixed solution 3 ~ 5ml, select a point next to the origin of the ophthalmosclerosis The sclera part penetrates, and then the fixative fluid is injected until the collapse of the eyeball occurs. Such as high eye pressure, eye