Using sensitive surface plasmon resonance to detect binding of peptide molecules and immobilized van

• 作 者: HE Yong-hui LIU Bin ZHAN Yan-feng LIU You-nian XIANG Juan
• 机 构: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
• 出 处: 中南大学学报(英文版)
• 发表日期: 2011年4期
• 关键词: flow injection surface plasmon resonance vancomycin Lys-Lys-Gly-D-Ala-D-Ala bind

To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peprides on the interface of solution and self-assemble monolayer,the binding between vancomycin and pentapeptide (Lys-Lys-Gly-D-Ala-D-Ala)was investigated by flow injection surface plasmon resonance (FI-SPR) and flow injection quartz crystal microbalance (FI-QCM).To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation,vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer.By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip,the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved (reaching 0.5×10-6 mol/L or 7.5×10-12 mol).The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained (KAds=5.0×104 L/mol).