解偶联剂(FCCP)与氰化钾(KCN)对禁食大鼠肝细胞酮体代谢的影响

• 作 者: 戴晨阳 吴洪章 李秀梅 张孝山 张妍 戴晨琳
• 机 构: 天津市传染病医院科研室,天津医科大学第三医院
• 出 处: 天津医科大学学报
• 发表日期: 1998年04期
• 关键词: 酮体 FCCP KCN 大鼠肝细胞 解偶联剂 丙酮酸 肝细胞损伤 阻断剂 呼吸链 作用机制

目的：研究解偶联剂（ＦＣＣＰ）和呼吸链阻断剂氰化钾（ＫＣＮ）对禁食大鼠肝细胞酮体代谢的影响。方法：使用胶原酶法分离大鼠肝细胞，然后分６组比较ＦＣＣＰ和ＫＣＮ对β羟丁酸（ＢＯＨＢ）和乙酰乙酸（ＡＣＡＣ）以及ＡＴＰ水平的影响。结果：单独使用ＦＣＣＰ和ＫＣＮ可使肝细胞损伤而酮体生成减少，加入丙酮酸后，ＦＣＣＰ使酮体生成（Ｐ＞００５），ＢＯＨＢ／ＡＣＡＣ比率与对照组无显著差别（Ｐ＞００５），而ＡＴＰ则明显减少（Ｐ＜００１）；ＫＣＮ则使酮体生成增加（Ｐ＜０００１），且ＢＯＨＢ／ＡＣＡＣ比率显著增加（Ｐ＜００１），ＡＴＰ也显著减少（Ｐ＜０００１）。结论：ＦＣＣＰ和ＫＣＮ均为强力的ＡＴＰ合成抑制剂，但作用机制不同，加入丙酮酸后ＦＣＣ使ＮＡＤＨ／ＮＡＤ比率降低，以ＡＣＡＣ为主；ＫＣＮ则使ＮＡＤＨ／ＮＡＤ比率增加，故以ＢＯＨＢ为主。

Objective: To study the effects of uncoupling agent (FCCP) and respiratory chain blocker potassium cyanide (KCN) on the metabolism of ketones in fasted rats. Methods: Rat hepatocytes were isolated by collagenase method and then compared the effects of FCCP and KCN on BOHB, ACAC and ATP levels in 6 groups. RESULTS: FCCP and KCN alone resulted in a decrease in hepatocyte injury and ketogenesis. After addition of pyruvate, FCCP resulted in ketone body formation (P> 005) and no significant difference in BOHB / ACAC ratio (P> 0) (P <001). KCN increased ketone body formation (P <0001), and the ratio of BOHB / ACAC increased significantly (P <001). ATP also markedly decreased Decrease (P <0001). CONCLUSION: Both FCCP and KCN are potent inhibitors of ATP synthesis, but their mechanism of action is different. After addition of pyruvate, the ratio of NADH / NAD decreases with the increase of NADH / NAD ratio and KAC increases the ratio of NADH / NAD, so BOHB is the main factor .