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目的探讨淫羊藿苷对人成骨样细胞(MG-63)成骨分化及OPG/RANKL表达的影响。方法用1 nmol/L、10 nmol/L、100 nmol/L、1μmol/L、10μmol/L 5种浓度的淫羊藿苷对MG-63进行干预,并同时进行成骨诱导,RT-PCR在第3天检测其细胞增殖基因MYC、CDK7表达量,在第6天检测成骨分化标志基因RUNX2、COL1A1及OPG、RANKL的表达量。结果对成骨分化相关基因,10 nmol、1μmol干预的MG-63 RUNX2基因表达量较1 nmol及10μmol的高(P<0.05),与100 nmol及空白对照组没有明显差别(P>0.05),10μmol的RUNX2蛋白表达量较其余各组更高;COL1A1的基因表达呈剂量依赖型,随浓度的增加而升高,10μmol的表达量较其余各组有明显升高(P<0.05),其蛋白表达也更多;RANKL的表达量极低,且在各组之间均无明显差异(P>0.05);OPG的表达在1μmol浓度时较100 nmol、10μmol明显增高(P<0.05),与1 nmol、10 nmol及空白组没有统计学差异(P>0.05);与细胞增殖相关的MYC的表达各组之间均没有差异(P>0.05),而CDK7的表达均较空白组降低(P<0.05)。结论 10μmol/L的淫羊藿苷能够明显促进MG-63细胞的成骨分化,但并不通过影响OPG/RANKL起效。
Objective To investigate the effects of icariin on osteoblast differentiation and expression of OPG / RANKL in human osteoblast-like cells (MG-63). Methods MG-63 was induced by icariin with concentrations of 1 nmol / L, 10 nmol / L, 100 nmol / L, 1 μmol / L and 10 μmol / L and osteogenic induction On day 3, the expression of MYC and CDK7 were detected. The expression of RUNX2, COL1A1, OPG and RANKL were detected on the 6th day. Results Compared with 100 nmol and blank control groups, there was no significant difference in the expression of RUNX2 gene between 10 nmol and 1 μmol groups compared with 1 nmol and 10 μmol groups (P> 0.05) 10μmol of RUNX2 protein expression higher than the other groups; COL1A1 gene expression in a dose-dependent manner, with increasing concentration increased 10μmol expression was significantly higher than the other groups (P <0.05), the protein (P> 0.05). The expression of OPG was significantly higher than that of 100 nmol and 10 μmol at the concentration of 1 μmol (P <0.05) nmol, 10 nmol and blank group had no statistical difference (P> 0.05). There was no significant difference in the expression of MYC between the groups (P> 0.05), while the expression of CDK7 was lower than that in the blank group (P < 0.05). Conclusion Icariin at a concentration of 10μmol / L can significantly promote the osteogenic differentiation of MG-63 cells without affecting the onset of OPG / RANKL.