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莫能菌素(Monensin)的菌丝体型和结晶型均曾在实验动物中试验过,其毒性机理可能与细胞内钾离子耗竭有关。两者的毒性类型和程度相似。现用菌丝体莫能菌素钠进行试验,其用量根据分析莫能菌素的活性而定,采取常见的口服法投药。试验步骤 1.急性毒性:用数种实验动物分组一次口服,观察毒性反应2周,计算出半数致死量(LD_(50))。 2.大鼠的三个月试验;3组4～5周龄Wistar大鼠(雌雄各15只),分别饲喂含莫能菌素钠50、150或500 ppm的饲料。另设一组作对照。每天逐一进行中毒检查,每周测定一次体重和饲料消耗量。试验结束前,分别采血样,进行血液学检查和血液生化值测定。最后尸检,对主要器官组织进行病理字检查。 3.狗的三个月试验:3组Beagle狗(雌雄各2只),
Monensin mycelia and crystal types have been tested in experimental animals, the toxicity mechanism may be related to intracellular potassium depletion. The type and extent of toxicity of both are similar. Now mycelium monensin sodium test, the amount according to the analysis of monensin activity may be, to take the common oral administration. Test Procedures 1. Acute Toxicity: Oral administration was given in groups of several experimental animals and the toxicity was observed for 2 weeks to calculate the median lethal dose (LD_ (50)). 2. Three-month rat test; Three Wistar rats, 4 to 5 weeks old, 15 male and 15 female, were fed with 50, 150, or 500 ppm of monensin respectively. Another set as a control. One by one poisoning check every week to determine the weight and feed consumption. Before the end of the experiment, blood samples were taken for hematological examination and determination of blood biochemical values. The final autopsy, the main organs of the pathological examination. Three-month test dogs: 3 Beagle dogs (male and 2 each)