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目的 观察高频喷射通气 (HFJV )治疗海水淹溺肺水肿 (PE- SWD)时兔球结膜微循环和肺 Na+ - K+ - ATP酶的变化 ,并探讨其变化机制。方法 向兔肺内灌入海水复制 PE- SWD动物模型。应用微循环显微镜和计算机图像分析系统 ,对不同处理两组兔球结膜微循环和肺毛细血管内皮细胞 Na+ -K+ - ATP酶进行自动检测和定量测量。结果 HFJV组球结膜微循环的面积、灰度和吸光度八项参数比PE- SWD组明显恢复。肺毛细血管内皮细胞 Na+ - K+ - ATP酶活力在 HFJV组中亦较 PE- SWD组明显升高 (P<0 .0 1或 P<0 .0 5 )。结论 HFJV可改善微循环和增加肺内 Na+ - K+ - ATP酶的活力 ,并与其有效地提高动脉血氧分压和纠正代谢性酸中毒密切相关。
Objective To observe the changes of rabbit conjunctiva microcirculation and lung Na + - K + - ATPase during HFJV treatment in seawater drowning pulmonary edema (PE-SWD) and to explore its mechanism. Methods Rabbit lungs were infused with seawater to replicate PE-SWD animal model. Microcirculation microscope and computerized image analysis system were used to detect and quantitatively measure conjunctival microcirculation and pulmonary capillary endothelial Na + -K + -ATPase in two groups. Results The area, gray level and absorbance of the conjunctival microcirculation in HFJV group were significantly higher than those in PE-SWD group. The activity of Na + - K + - ATPase in pulmonary capillary endothelial cells in HFJV group was also significantly higher than that in PE-SWD group (P <0.01 or P <0.05). Conclusion HFJV can improve microcirculation and increase the activity of Na + - K + - ATPase in the lung, which is closely related to the improvement of arterial partial pressure of oxygen and correct metabolic acidosis.