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目的:观察灯盏花素对糖尿病大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖及核转录因子-κB(NF-κB)的影响。方法:建立链脲佐菌素糖尿病大鼠模型,用组织贴壁法体外培养糖尿病及正常VSMCs,通过细胞计数试剂盒(cellcounting kit-8,CCK-8)测定灯盏花素对糖尿病及正常VSMCs的50%抑制率浓度(IC50),采用免疫荧光-共聚焦显微镜检测灯盏花素对糖尿病VSMCs中NF-κB核转运影响。结果:灯盏花素能抑制糖尿病及正常VSMCs的增殖,其IC50分别为8.63±0.69 mg/L、31.61±3.90 mg/L;不同浓度的灯盏花素干预糖尿病VSMCs 24h后明显抑制NF-κB的激活,减少核转运。结论:灯盏花素对糖尿病VSMCs增殖抑制作用强于正常VSMCs,其机制可能与下调糖尿病VSMCs的NF-κB核转运有关。
Objective: To observe the effect of breviscapin on proliferation and nuclear factor-κB (NF-κB) in vascular smooth muscle cells (VSMCs) of diabetic rats. Methods: The streptozotocin-induced diabetic rats were established. Diabetes mellitus and normal VSMCs were cultured in vitro by tissue adherent method. The effects of breviscapine on diabetic and normal VSMCs were determined by cell counting kit-8 (CCK-8) 50% inhibitory concentration (IC50). The effect of breviscapine on NF-κB nuclear translocation in diabetic VSMCs was detected by using immunofluorescence-confocal microscopy. Results: Breviscapine could inhibit the proliferation of diabetic and normal VSMCs with the IC50 of 8.63 ± 0.69 mg / L and 31.61 ± 3.90 mg / L, respectively. Breviscapine significantly inhibited the activation of NF-κB in diabetic VSMCs for 24h , Reduce nuclear translocation. Conclusion: Breviscapine can inhibit the proliferation of diabetic VSMCs more than normal VSMCs, which may be related to the down-regulation of nuclear translocation of NF-κB in diabetic VSMCs.