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目的体外观察人类免疫缺陷病毒-1(human immunodeficiency virus-1,HIV-1)膜蛋白gp120 V3环的神经毒性。方法通过小鼠大脑皮质原代神经细胞培养条件下TUNEL检测和培养上清乳酸脱氢酶释放试验,结合抗微管蛋白-2(microtubule associated protein-2,MAP-2)免疫荧光试验,观察人重组gp120蛋白神经毒性,并通过蛋白印迹法初步探讨其与凋亡调控蛋白bax与bcl-2的关系。结果重组人gp120 V3环多肽可诱导神经元凋亡和抑制神经突起形成,并呈浓度依赖性。gp120 V3环诱导神经细胞凋亡与抑制bcl-2表达有关。结论 HIV-1膜蛋白gp120 V3环具有神经毒性。
Objective To observe the neurotoxicity of gp120 V3 loop of human immunodeficiency virus-1 (HIV-1) membrane protein in vitro. Methods TUNEL assay and culture supernatant of lactate dehydrogenase (LDH) release assay were performed in mouse primary cortical neuronal cultures. Combined with microtubule associated protein-2 (MAP-2) immunofluorescence assay, human The recombinant gp120 protein was neurotoxic. The relationship between bax and bcl-2 was analyzed by Western blotting. Results Recombinant human gp120 V3 loop peptide could induce neuronal apoptosis and inhibit neurite formation in a concentration-dependent manner. Gp120 V3 loop induces neuronal apoptosis and inhibits bcl-2 expression. Conclusion The HIV-1 membrane protein gp120 V3 loop is neurotoxic.