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目的:研究运动训练与脑出血大鼠脑组织中白细胞介素-10(IL-10)和半胱氨酸天冬氨酸蛋白酶-3(caspase-3)的相互关系,探讨运动训练促进脑出血(ICH)后神经功能恢复的机制。方法:采用自体血注入法将80只SD大鼠制作成右侧纹状体脑出血模型,造模成功后按照随机化的原则将其分为对照组和运动训练组(运动组),每组又分为第1、3、7、14、21天5个时间点。运动组行网屏训练、平衡木训练、滚笼训练,对照组不作任何干预。分别于不同时间点对大鼠进行神经功能评分。然后将其处死,取右侧脑组织用免疫组化和原位杂交的方法检测IL-10和caspase3的蛋白及mRNA表达。结果:在脑出血后第14天和第21天运动组大鼠的神经功能评分优于对照组,运动组大鼠的IL-10表达高于对照组;在脑出血后第7天运动组大鼠的caspase3表达低于对照组。结论:运动训练可使脑出血大鼠脑组织内IL-10含量增高,而使caspase-3的含量降低,这可能是运动训练促进神经功能的恢复机制之一。
Objective: To investigate the relationship between interleukin-10 (IL-10) and caspase-3 in brain tissue of rats with exercise-induced and intracerebral hemorrhage (ICH) and to explore whether exercise training can promote cerebral hemorrhage (ICH) after the nerve function recovery mechanism. Methods: Eighty SD rats were made into right cerebral striatum by autologous blood injection. According to the principle of randomization, they were divided into control group and exercise training group (exercise group) Also divided into 1,3,7,14,21 days 5 time points. Exercise group line screen training, balance beam training, cage training, the control group without any intervention. Neurological scores were evaluated at different time points. Then they were sacrificed, and the right brain tissue was used to detect the protein and mRNA expression of IL-10 and caspase3 by immunohistochemistry and in situ hybridization. Results: The neurological score of exercise group was better than that of control group on the 14th day and the 21st day after cerebral hemorrhage. The expression of IL-10 in exercise group was higher than that of the control group. On the 7th day after cerebral hemorrhage, Mouse caspase3 expression was lower than the control group. Conclusion: Exercise training can increase the content of IL-10 in the brain tissue of rats with intracerebral hemorrhage and decrease the content of caspase-3, which may be one of the mechanisms of recovery from motor training.